Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g 2nd edition ed

2nd edition ed

2 at 25°C Suspend 50 gram powdered medium in 1 litre distilled water Store dry at room temperature

2 Glucose 20 Tween 80 1 Sodium Acetate   5 Dipotassium Hydrogen Phosphate  2 Manganese sulphate    0

Malt Extract 30 Adenine 0

respectively 10% and 5% of the NaCl concentration is present in both formulations

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g 2nd edition edCalcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room