TBS Buffered Saline 10X, pH 7.4 Size:1L 35 mM) of KCl is

35 mM) of KCl is added

Folic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin

Current Protocols

quick and no pH adjustment necessary

mineral salts and trace elements required for a vigorous growth of yeast cells

TBS Buffered Saline 10X, pH 7.4 Size:1L 35 mM) of KCl is10 X sterile stock solution Dilute 100ml TBS Buffered Saline 10X into 900ml deionised water to make 1 litre of TBS Saline Buffer 1 X. Final concentration is 0. 1 M Tris Buffer and 0. 14 M Sodium chloride, pH 7. 4. Specifications TRIS Buffer 1. 0 M 121. 4 g L Sodium Chloride 1. 4 M 81. 9 g L Store at room temperature Keep away from light